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KMID : 1134820100390070960
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Journal of the Korean Society of Food Science and Nutrition
2010 Volume.39 No. 7 p.960 ~ p.965
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Antitumor Activity of Corni Fructus Ethanol Extract in Sarcoma-180 Cancer Cells
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Kwon Seong-Hyuk
Seo Kwon-Il
Lee Mi-Kyung
Kang Kap-Suk
Kwon Soon-Jae
Kim Jae-Yong
Shim Ki-Hwan
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Abstract
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To develop Corni Fructus as a cancer preventive food material, the in vitro cytotoxicities and in vivo antitumor activities of various concentrations of 80% Corni Fructus ethanol extract (CFEE) were investigated using sarcoma-180 cancer cell. Viability was decreased and cell death rate was increased in both dose- and time-dependent manners in cells treated with CFEE at 10, 100, 300, and 500 ㎍/㎖ concentrations for 24, 48, and 72 hr. Proliferation was also inhibited more than 60% in cells treated with CFEE at the 100 ㎍/㎖ concentration for 48 hr. In addition, the morphology of cells treated with CFEE at the 100 and 500 ㎍/㎖ concentrations was distorted with shrunken cell masses and lower cell numbers compared to the control cells. In the cells treated with CFEE, the formation of apoptotic bodies and nuclear condensation were observed in dose dependent manners. CFEE also increased DNA fragmentation values at the 100 and 500 ㎍/㎖ concentrations. The apoptosis induced by CFEE was connected to the proteolytic activation of caspase-3. When CFEE was administered at 100 and 300 ㎎/㎏, ip, for 7 consecutive days in mice inoculated with sarcoma-180 cancer cell, the life span of the mice was found to be longer than that of the control mice that did not receive the extract. These results suggest that Corni Fructus may be used as a potential cancer preventive food material.
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KEYWORD
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Corni Fructus, apoptosis, antitumor activity, sarcoma-180
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